User Name :
Password :
Administrator Login   

TRUPCR BCR ABL Kit

One step Qualitative detection of BCR-ABL transcript

The TRUPCR BCR-ABL REAL TIME PCR KIT is intended for the qualitative detection and differentiation of BCR-ABL fusion gene transcript Major (M), minor (m), and micro (µ) in bone marrow or peripheral blood samples of acute lymphoblastic leukemia(ALL) or chronic myeloid leukemia (CML) using real time PCR system.  

The TRUPCR BCR-ABL REAL TIME PCR KIT has two-step protocol in which total RNA is reverse-transcribed, and the generated cDNA is amplified by PCR using a pair of specific primers and a specific internal double-dye probe of BCR ABL (Major, Minor and Micro) and ABL. The probe binds to the amplicon during each annealing step of the PCR. When the Taq extends from the primer bound to the amplicon, it displaces the 5' end of the probe, which is then degraded by the 5'-3' exonuclease activity of the Taq DNA polymerase. Cleavage continues until the remaining probe melts off the amplicon. This process releases the fluorophore and quencher into solution, spatially separating them and leading to an increase in fluorescence from the reporter and a decrease in fluorescence from the quencher.

Product Feature

  • Detection of all known BCR ABL fusion transcripts i.e. Major, Minor and Micro.
  • Rapid, accurate & detection of all fusion transcripts in separate tubes.
  •  Higher diagnostics sensitivity and specificity.
  • All the reagents required (cDNA preparation to PCR) for the test included in the kit
  •  Compatible with all open real time PCR equipment

Ordering Information
ProductFormatCat No. 
TRUPCR BCR ABL Kit 48 RXN 3B261.1
TRUPCR BCR ABL Kit 96 RXN 3B261.2
Home About 3B Diagnostics R & D Career Sitemap Contact Us
Powered By :Business Buddies
All Right Reserved-3B BlackBio Biotech India Limited