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Quantitative Detection of BCR-ABL transcript

The TRUPCR BCR-ABL REAL TIME PCR KIT is intended for the quantitative detection and differentiation of BCR-ABL fusion gene transcript Major (M), minor (m), and micro (µ) in bone marrow or peripheral blood samples of acute lymphoblastic leukemia(ALL) or chronic myeloid leukemia (CML) using real time PCR system.  This kit evaluates the level of molecular response in international scale (IS); results can be used for minimal residual disease follow-up.

The TRUPCR BCR-ABL REAL TIME PCR KIT is designed according to the “Europe Against Cancer” (EAC) and Guidelines for the measurement of BCR-ABL1transcripts in chronic myeloid leukaemia with the updated international recommendations. For reporting in international scale (IS) this kit is calibrated against First WHO International Genetic Reference Panel for quantitation of BCR-ABL translocation by RQ-PCR (NIBSC code: 09/138).

The TRUPCR BCR-ABL REAL TIME PCR KIT has two-step protocol in which total RNA is reverse-transcribed, and the generated cDNA is amplified by PCR using a pair of specific primers and a specific internal double-dye probe of BCR ABL (Major, Minor and Micro) and ABL. The probe binds to the amplicon during each annealing step of the PCR. When the Taq extends from the primer bound to the amplicon, it displaces the 5' end of the probe, which is then degraded by the 5'-3' exonuclease activity of the Taq DNA polymerase. Cleavage continues until the remaining probe melts off the amplicon. This process releases the fluorophore and quencher into solution, spatially separating them and leading to an increase in fluorescence from the reporter and a decrease in fluorescence from the quencher. 

Product Feature

  • Detection of all known BCR ABL fusion transcripts i.e. Major, Minor and Micro
  • Rapid, accurate & detection of all fusion transcripts in separate tubes
  • Higher diagnostics sensitivity, up to as ≥ 4.5 log reduction of BCR-ABL on the international scale and can be used for minimal residual disease follow-up.
  • All the reagents required (cDNA preparation to PCR) for the test included in the kit
  • Performance compatible and comparable to the International Scale

Ordering Information
ProductFormatCat No. 
48 RXN 3B1202.1
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