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TRUPCR DENV Kit is a Real-Time Amplification test for the qualitative/quantitative detection of Dengue Virus RNA from human serum or plasma, encompassing all the major Dengue viral genotype. RNA is extracted from samples, amplified using Real Time Amplification and detected using fluorescent reporter dye probes specific for Dengue virus genotypes. In real-time PCR, the fluorescent signal is generated from the presence of an oligonucleotide probe specific for target DNA sequence. The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During synthesis of a complementary chain, Taq DNA polymerase which possesses 5' - 3' exonuclease activity cleaves the probe. As a result, the fluorescent dye and quencher dye are separated, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of reaction, and the threshold cycle value is determined from the obtained curve. The threshold cycle is proportional to the initial number of DNA copies in a sample and its value allows qualitative/quantitative comparisons of analyzed and control samples.

TRUPCR DENV Qualitative Kit is designed to detect all four serotypes of dengue virus (DEN1, DEN2, DEN3 and DEN4).

Ordering Information
ProductFormatCat No. 
TRUPCR Dengue Kit 48 RXN 3B253
TRUPCR Dengue Kit  96 RXN 3B254
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