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TRUPCR HIV Viral Load Kit

TRUPCR HIV Viral Load Kit is a Real-Time Amplification test for the qualitative/quantitative detection of Human Immunodeficiency Virus type I RNA in human serum or plasma. RNA is extracted from samples, reverse transcribed using RT-PCR and the obtained cDNA is then amplified using Real Time Amplification and detected using fluorescent reporter dye probes specific for HIV type I. In real-time PCR, the fluorescent signal is generated from the presence of an oligonucleotide probe specific for target DNA sequence. The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During synthesis of a complementary chain, Taq DNA polymerase which possesses 5' - 3' exonuclease activity cleaves the probe. As a result, the fluorescent dye and quencher dye are separated, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of reaction, and the threshold cycle value is determined from the obtained curve. The threshold cycle is proportional to the initial number of RNA copies in a sample and its value allows qualitative/quantitative comparisons of analyzed and control samples.

TRUPCR HIV Viral Load kit is based on amplification of conserved region by primer and probes specific for HIV type I. In this kit there are two independent reactions running in parallel in each tube: the first detects HIV type I (FAM channel), second detects internal control (IC) DNA (HEX/VIC channel) which allows excluding unreliable results.

Ordering Information
ProductFormatCat No. 
TRUPCR HIV Viral Load kit 48 RXN 3B257
TRUPCR HIV Viral Load Kit 96 RXN 3B258
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