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TRUPCR HPV 16 & 18 Kit

TRUPCR HPV 16&18 is a Real-Time Amplification test for the qualitative detection of Human Papilloma Virus DNA in clinical samples. DNA is extracted from samples and is then amplified using Real Time Amplification and detected using fluorescent reporter dye probes specific for HPV 16&18. In real-time PCR, the fluorescent signal is generated from the presence of an oligonucleotide probe specific for target DNA sequence. The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During synthesis of a complementary chain, Taq DNA polymerase which possesses 5' - 3' exonuclease activity cleaves the probe. As a result, the fluorescent dye and quencher dye are separated, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of reaction, and the threshold cycle value is determined from the obtained curve. The threshold cycle is proportional to the initial number of DNA copies in a sample and its value allows qualitative comparisons of analyzed and control samples.

TRUPCR HPV 16&18 detection kit is based on amplification of conserved region by primer and probes specific for HPV 16&18. In this kit there are three independent reactions running in parallel in two tube: the first detects HPV 16 (FAM channel), and internal control (IC) DNA (Texas Red) which allows excluding unreliable results and second detects HPV18 (HEX channel).

Ordering Information
ProductFormatCat No. 
TRUPCR HPV 16 & 18 Kit 48 RXN 3B1210.1
TRUPCR HPV 16 & 18 Kit 96 RXN 3B1210.2
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