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Pfu DNA Polymerase 1U/μl Download Manual

3B Pfu DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3’-5’ exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases). 

3B Pfu DNA Polymerase generates blunt-ended amplification products, enabling their cloning into blunt-ended vectors. It is suitable for applications including: 

  • Cloning

  • Mutagenesis

  • Achieving greater fidelity than with Taq DNA polymerase

  • Amplifying fragments of up to 5 kd

3B Pfu DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer including 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.
Ordering Information
ProductFormatCat No. 
3B Pfu DNA Polymerase (1U/µl) – Standard Reaction Buffer 100 U 3B047
3B Pfu DNA Polymerase (1U/µl) – Standard Reaction Buffer 250 U 3B048
3B Pfu DNA Polymerase (1U/µl) – Reaction Buffer MgCl2 Free 250 U 3B052
3B Pfu DNA Polymerase (1U/µl) – Reaction Buffer MgCl2 Free 100 U 3B051
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