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Quantimix Easy Syg Kit Download Manual

QUANTIMIX EASY KIT has been optimized to deliver maximum efficiency, precision, and sensitivity during nucleic acid amplification in real time using CHROMOFY™.


Real time detection of amplified products is performed by monitoring the increase of fluorescence after each amplification cycle. In QUANTIMIX EASY KIT the fluorescent signal is generated by the incorporation of CHROMOFY™ to the amplified products. In the exponential phase of the amplification there is a correlation between the amount of product and the initial template DNA and the amount of fluorescence is proportional to the amplified DNA in each cycle. The real time detection of amplified products is performed by monitoring the increase of fluorescence after each amplification cycle. The fluorescent signal is generated by the incorporation of Chromofy, a third generation dye developed by TATAA Biocenter, that has absorbance and emission wavelengths suitable for the FAM/SYBR channel.


QUANTIMIX EASY KIT is a ready-to-use 2X Master Mix (QUANTIMIX EASY MASTERMIX), which contains: 3B DNA polymerase, all four dNTPs, MgCl, and reaction buffer. The easy-to-use Master Mix reduces set up time and liquid handling steps. All reaction components are included in the QUANTIMIX EASY MASTERMIX, except template; primers; and CHROMOFY™


The kit is compatible with real-time PCR thermal cyclers that use either standard or capillary reaction vials.
  • QUANTIMIX EASY MASTERMIX: It is a ready-to-use 2X solution that contains , necessary components for real time amplification assays:3B DNA Polymerase, dNTPs, Reaction Buffer, and MgCl2 is also included at the appropriate concentration (4mM).
  • Chromofy™: It is supplied as a stock solution in DMSO.
  • 50 mM MgCl2 Solution: Used only for specific real time assays which require an additional optimization.
  • qPCR Astringent: Used for specific real-time PCR assays, increase the sensitivity. Optional use.
Ordering Information
ProductFormatCat No. 
Quantimix Easy kit 100 rxn 3B101
Quantimix Easy kit 200 rxn 3B102
Quantimix Easy kit 500 rxn 3B103
Quantimix Easy kit 1000 rxn 3B104
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