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3B High Retrotranscriptase Download Manual

3B BlackBio Biotech High Retrotranscriptase is a thermostable, new recombinant reverse transcriptase expressed in E. coli.

The enzyme is an RNase H minus which exhibits high affinity for RNA and works in a higher range of temperature than most commercially retrotranscriptases. Due to its thermostability, 3B High Retrotranscriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.

3B High Retrotranscriptase will accept three different types of primer for cDNA synthesis: Oligo (dT) primer; random hexamer primers; or a gene-specific primer.

The enzyme can be used at a temperature up to 65°C, and it is recommended for RT-PCR or qRT-PCR.

Product applications:

·         Synthesis of first strand cDNA for use in subsequent amplification reactions: RT-PCR or qRT-PCR.

·         RT-PCR amplification of difficult RNA templates, such as GC-rich templates with large amounts of secondary structure

·         Incorporation of Cy3-, Cy5-, Biotin-, or aminoallyl-modified nucleotides during cDNA synthesis (e.g., for use in microarray hybridization).

·         3B BlackBio Biotech High Retrotranscriptase: Provided in storage buffer. Storage buffer: 5 mM Tris-Acetate (pH 8.0), 15 mM  KCl, 1.5 mM EDTA, protease inhibitors, and 50% glycerol (v/v).
·         10X High RT Reaction Buffer: 100 mM Tris-HCl (pH 9.0), 500 mM KCl, and 1 mM  DTT.
·         100 mM MgSO4 Solution

Ordering Information
ProductFormatCat No. 
3B High Retrotranscriptase 100 rxn 3B120
3B High Retrotranscriptase 500 rxn 3B121
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