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TRUPCR® CHIKUNGUNYA RT KIT
TRUPCR® CHIKUNGUNYA RT KIT accurately detects Chikungunya Virus (CHIKV) in a qualitative form from various sources of clinical samples using Real Time PCR technique.


TRUPCR® Chikungunya Kit is a Real-Time Amplification test for the qualitative detection of Chikungunya Virus (CHIKV) RNA from human serum or plasma, encompassing all the Chikungunya viral genotype. RNA is extracted from samples, amplified using Real Time Amplification and detected using fluorescent reporter dye probes specific for Chikungunya virus genotypes. In real-time PCR, the fluorescent signal is generated from the presence of an oligonucleotide probe specific for target DNA sequence. The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During synthesis of a complementary chain, Taq DNA polymerase which possesses 5' - 3' exonuclease activity cleaves the probe. As a result, the fluorescent dye and quencher dye are separated, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of reaction, and the threshold cycle value is determined from the obtained curve. The threshold cycle is proportional to the initial number of RNA copies in a sample and its value allows qualitative comparisons of analyzed and control samples.


Advantages:
  • One step Real Time PCR kit- Includes all reagents for test
  • Higher sensitivity and specificity
  • Provided with internal control to exclude unreliable results
  • Compatible with different instruments

Ordering Information
ProductFormatCat No. 
TRUPCR® CHIKUNGUNYA RT KIT     (Real Time)
48 RXN 3B1240
TRUPCR® CHIKUNGUNYA RT KIT (Real Time)
96 RXN 3B1241
TRUPCR® CHIKUNGUNYA RT KIT      (End Point)   
48 RXN 3B1242
TRUPCR® CHIKUNGUNYA RT KIT  (End Point)   

96 RXN 3B1243
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