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3B PCR Mastermixes - Final Time

3B PCR Master Mix is a 2x concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation is timing-saving and minimises contamination due to a reduced number of handling steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Key Features:

  • High throughput PCR- To perform PCR, only the addition of a template and primers is required.
  • The 3B 2x PCR Master Mix can be used with conventional PCR machines.
  • Routine PCR with High Reproducibility.
  • Generation of PCR products for TA Cloning.
  • cDNA amplification

Ordering Information:

CAT. NO. PRODUCT FORMAT
3B089 3B PCR Master Mix (4 x 1.25 ml) 200 Reactions
3B090 3B PCR Master Mix (10 x 1.25 ml) 500 Reactions
3B091 3B PCR Master Mix (20 x 1.25 ml) 1000 Reactions

3B Directload PCR Master Mix

3B Directload Master Mix is a ready to use 2x concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. The mastermix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. This pre-mixed formulation is timing-saving and minimises contamination due to a reduced number of handling steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Key Features:

  • Exceptional value in terms of cost per unit.
  • 2x master mix format for easy reaction setup- just add template DNA and primers.
  • Offered with inert tracking dyes for easy and direct loading of PCR products onto gels.

Ordering Information:

3B Direct Load PCR Master Mix

CAT. NO. PRODUCT FORMAT
3B093 3B Directload PCR Master Mix (4 x 1.25 ml) 200 Reactions
3B094 3B Directload PCR Master Mix (10 x 1.25 ml) 500 Reactions
3B095 3B Directload PCR Master Mix (20 x 1.25 ml) 1000 Reactions

3B Hotstart PCR Master Mix

3B Hotstart PCR Master Mix is a 2x concentrated solution of 3B HotStart DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation is timing-saving and minimises contamination due to a reduced number of handling steps required for PCR set up. The mix is optimized for efficient and reproducible PCR. This formulation provides the following features to the HotStart DNA Polymerase.

  • Enhanced enzyme specificity and sensitivity.
  • Increase the obtained yield.
  • Reduce mis-priming and primer-dimer formation.

Applications:

  • Standard PCR
  • Multiplex PCR
  • DNA labelling
  • Difficult PCR because the target sequence has a low copy number.
  • Problematic PCR reactions due to primer-dimers.

Ordering Information:

CAT. NO. PRODUCT FORMAT
3B097 3B Hotstart PCR Master Mix (2 x 1.25 ml) 100 Reactions
3B098 3B Hotstart PCR Master Mix (4 x 1.25 ml) 200 Reactions
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