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3B REVERSE TRANSCRIPTASE

3B Reverse Transcriptase is a thermostable, new recombinant reverse transcriptase expressed in E. coli. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity. The enzyme is an RNase H minus which exhibits high affinity for RNA and works in a higher range of temperature than most commercially reverse transcriptases. Due to its thermostability, 3B High Retrotranscriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.

Key Features:

  • Optimum activity at 42°C and the enzyme is active up to 50°C.
  • Efficient synthesis of cDNA up to 13 kb.
  • Incorporation of Cy3-, Cy5-, Biotin-, or aminoallyl-modified nucleotides during cDNA synthesis (e.g., for use in microarray hybridization).

Product Application:

  • Synthesis of first strand cDNA for use in subsequent amplification reactions: RT-PCR or qRT-PCR.
  • Synthesis of cDNA for cloning and gene expression.
  • RT-PCR amplification of difficult RNA templates, such as GC-rich templates with large amounts of secondary structure.
  • Labeled cDNA probes generation for microarrays.

Ordering Information:

CAT. NO. PRODUCT FORMAT
3B120 3B REVERSE TRANSCRIPTASE 100 Reactions
3B121 3B REVERSE TRANSCRIPTASE 500 Reactions

3B REVERSE TRANSCRIPTASE – STARTER KIT

3B Reverse Transcriptase-Starter Kit is a complete system for efficient synthesis of first strand cDNA. The kit uses the Reverse transcriptase, a new reverse transcriptase that features a high thermostability and lacks the RNase H activity. The enzyme provides first strand cDNA synthesis with a broader optimal reaction temperature from 40°C to 65°C. Due to its thermostability, reverse transcriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.

Key Features:

  • Complete kit for cDNA synthesis- Includes optimized buffer and dNTP mix, Random Primer and RNase Inhibitor.
  • High yield of full- length cDNA up to 13kb.
  • Increased activity of enzyme in the range of 42 to 55°C.

Product Application:

  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR.
  • Construction of full length cDNA libraries.
  • Synthesis of cDNA for cloning and gene expression.

Ordering Information:

CAT. NO. PRODUCT FORMAT
3B122 3B REVERSE TRANSCRIPTASE – STARTER KIT 50 Reactions
3B123 3B REVERSE TRANSCRIPTASE – STARTER KIT 100 Reactions
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