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MTB Extraction

TRUPCR® MTB DNA Extraction Kit is intended for the extraction and purification of DNA from M.tuberculosis spp. in human samples (sputum, BAL, PUS, tissues and other body fluids).
TRUPCR® MTB DNA Extraction Kit is based on the advanced silica binding technology. The DNA extraction procedure includes 4 steps:

Heat Lysis- Lysis of the bacterial cell wall at high temperature and release of DNA.
Binding- Adsorption of the DNA on to the membrane.
Washing- Removal of residual contaminants.
Elution- Elution of pure genomic DNA.


Key Features:

  • Based on Silica column technology, reduces the hands on procedure time.
  • TRUPCR® MTB DNA Extraction kit is validated on all clinical specimens including sputum, PUS, BAL, tissue, CSF and other body fluids.
  • Phenol chloroform free method.
  • High binding capacity of the column provides high yield of purified DNA.

Specification:

Technology Silica Column
Sample Type Sputum, Pus, BAL, Tissue, Urine, Body fluids and tissue
Application PCR and Real Time PCR
Elution Volume 40-50 µl
Compatible with TRUPCR® MTBC Onestep Nested kit and TRUPCR® Rifampicin Resistance MTB Detection Kit
Processing Manual (Centrifugation)

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B268E TRUPCR MTB DNA Extraction Kit 50/100 Rxn
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