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TRUPCR® EGFR Mutation Kit

The TRUPCR® EGFR Mutation Kit is an in vitro diagnostic test intended for the qualitative detection of EGFR somatic mutations in the genomic DNA extracted from fresh, frozen or formalin fixed paraffin-embedded (FFPE) tissue. The TRUPCR® EGFR mutation kit is based on allele specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The kit is designed to selectively amplify mutant specific sequences in samples that contain a mixture of wild-type and mutated DNA. The most common mutations are found in exons 19 and 21. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® EGFR Kit is composed of 9 assays for the detection of the EGFR mutations and a reference control gene of EGFR region without any known polymorphism / mutation.

Key Features:

  • Selective Amplification of DNA containing mutation with ARMS Technology.
  • Sensitive to detect up to 1% mutation in EGFR gene.
  • Detects 32 different mutations in a single run.
  • Extraction control included to avoid false-negative results.
  • Rapid, more reliable, comprehensive and cost-effective tests.
  • Easy work flow & compatible with various Real Time PCR instruments.

EGFR Detectable mutations:

EXON CODON MUTATION NUCLEOTIDE CHANGE
18 719 G719S c.2155 G>A
G719C c.2155 G>T
G719A c.2155 G>C
20 790 T790M c.2369 C>T
768 S768I c.2303 G>T
797 C797S c.2390G>C
797 C797S c.2389T>A
769_770 V769_D770insASV 2307_2308insGCCAGCGTG
770_771 D770_N771insG 2310_2311insGGT
773_774 H773_V774insH 2319_2320insCAC
21 858 L858R c.2573 T>G
861 L861Q c.2582 T>A
19 746_750 E746_A750del 2235_2249del15)
746_750 E746_A750del 2236_2250del15)
747_753 L747_P753>S 2240_2257del18)
747_750 L747_A750>P 2239_2248TTAAGAGAAG>C)
746_752 E746_S752>V 2237_2255>T
747_751 L747_T751del 2240_2254del15
747_752 L747_S752del 2239_2256del18
746_751 E746_T751>A 2237_2251del15
747_751 L747_T751del 2239_2253del15
747_751 L747_T751>P 2239_2251>C
747_749 L747_E749del 2239_2247del9
746_749 E746_E749del 2235_2246del12
747_753 L747_P753>Q 2239_2258>CA
747_751 L747_T751>S 2240_2251del12
746_752 E746_S752>A 2237_2254del18
747_750 L747_A750>P 2238_2248>GC
746_752 E746_S752>D 2238_2255del18
746_751 E746_T751>I 2235_2252>AAT
747_751 L747_T751>Q 2238_2252>GCA
746_751 E746_T751del 2236_2253del18


This kit can become useful in suggesting that all patients to be administered anti-EGFR monoclonal antibody therapy, should first be screened for EGFR mutations.

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B1311 TRUPCR® EGFR Mutation Kit 48 Rxn
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