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TRUPCR® PML - RARA PCR Kit

Detection of the PML-RARA t(15;17) translocation is diagnostic for acute promyelocytic leukemia (APL), although the diagnosis can also be based on morphology. Investigations suggest that 99% of APL patients harbor a translocation between chromosomes 15 and 17, which fuses the retinoic acid receptor alpha (RARA) gene on chromosome 17 with the PML gene on chromosome 15. Detection of the PML-RARA t(15;17) translocation is therefore used within clinical research as an identifier for APL. Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed. They represent 50-55%, 5-10% and 30-40% of the cases respectively.
TRUPCR® PML-RARA is an in-vitro nucleic acid amplification assay for the qualitative detection of PML-RAR-alpha fusion transcripts in human clinical samples. In this multi-tube assay, extracted RNA is subjected to a separate real-time reverse transcription-polymerase chain reaction (RT-PCR) procedures to detect long (L or bcr1), variant (V or bcr2) and short (S or bcr3) isoforms simultaneously. An additional amplification for the ABL gene is performed as a control for sample RNA quality.


Key Features:

  • First commercial assay to accurately detect and differentiate of bcr1, bcr2 & bcr3 fusion transcripts
  • Detection and differentiation of both 5’ and 3’ forms of bcr2 (variant) form.
  • Higher sensitivity and specificity with easy workflow and quick analysis.
  • All the reagents required for the test included in the kit.
  • Compatible with various real time PCR instruments.

Ordering Information:

CAT. NO. PRODUCT CONTENTS
3B1258 TRUPCR® PML-RARA Qualitative Kit 24 Rxn
3B1255 TRUPCR® PML-RARA Qualitative Kit 48 Rxn
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