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Thrombophilia Panel Kit

TRUPCR® Coagulation Panel Kit.

The TRUPCR® Coagulation Panel Kit is designed for the qualitative detection of Factor-II Mutation (Prothrombin, G20210A), Factor-V Leiden Mutation (G1691A) and MTHFR Mutation (C677T and A1298C) in human blood.

TRUPCR® Coagulation Panel Kit is an allelic discrimination real-time polymerase chain reaction (PCR) assay for qualitative detection of the mutation against a background of wild-type genomic DNA. In an allelic discrimination, two different probes specific for each allele are included in the PCR assay. Each probe is labelled with a different fluorescent dye (such as FAM or HEX/VIC) at its 5' end and contains a non fluorescent quencher at the 3' end. During qPCR amplification of the target DNA the probes will compete for binding across the variant region. The probe that is 100% homologous to the DNA binding site will preferentially bind and give a fluorescent signal as PCR proceeds. It follows that the wild-type sequence will give a strong amplification plot through one channel whilst giving a very weak signal through the alternative channel. Homozygous variant samples will give an exactly inverse result. A mismatch between probe and target greatly reduces the efficiency of probe hybridization and cleavage and no reporter dye is released. Thus, substantial increase in FAM or HEX/VIC dye fluorescence indicates homozygosity for the FAM- or HEX/VIC- specific allele. An increase in both signals indicates heterozygosity.

  • Thrombophilia Panel Kit
  • Thrombophilia Panel Kit

Key Features:

  • Detects Factor II Mutation (Prothrombin, G20210A), Factor V Leiden Mutation (G1691A) and MTHFR Mutation (C677T and A1298C) in single assay.
  • Rapid and extremely accurate tests.
  • Based on allelic discrimination, its more reliable and highly reproducible assay.
  • Higher sensitivity and specificity.
  • Amplification of wild type allele to check DNA Quality and Quantity.
  • Easy work flow & compatible with Applied Biosystems 7300 / 7500 Real-Time PCR System, AriaMx Real-Time PCR System, CFX ConnectTM / CFX96TM / Dx Real-Time PCR Detection System, QuantStudioTM 3 and 5 Real-Time PCR System, Rotor-Gene Q, StepOneTM / StepOnePlusTM Real-Time PCR System.

Ordering Information:

3B1400 TRUPCR® Coagulation/ Thrombophilia Panel Kit 24 Rxns
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