3B DNA Polymerase is a modified thermostable recombinant DNA polymerase from the thermophilic bacterium Thermus sp. expressed in E. coli. The general characteristics of 3B DNA Polymerase make the enzyme suitable for applications requiring a highly thermostable and processive enzyme capable of synthesizing DNA strands at elevated temperatures in amplification or similar reactions (e.g. primer extension), thus resolving the most complex secondary structures.
Due to its processivity and accuracy 3B DNA Polymerase allows the generation of long templates with a base misincorporation rate (1-10 x 10-6 bp) lower than most commercial Taq DNA polymerases.
The procedure employed for the purification of thermostable enzymes is proprietary of 3B BlackBio Biotech India Limited. It involves a simple and non chromatographic procedure which renders a top high yield and quality enzyme.
3B DNA Polymerase (1U/µl, 3U/µl and 5U/µl) comes with the choice of a Standard Reaction Buffer including 20 mM MgCl2 or a reaction buffer without MgCl2 (Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.
|Buffers||Supplies with two different buffers combination and separate MgCl2 to allow PCR for wide range of magnesium concentration|
|Stability||Stable at higher temperature|
|Application||Standard PCR and Real Time PCR, Generates PCR products with 3'-dA overhangs|
|DNA Labelling||Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)|
3B Hotstart DNA Polymerase has been designed and developed to overcome the problems due to non-specific priming, primer- dimer formation or other unwanted reactions occuring at low temperature during the PCR process.
The technology behind 3B Hot Start DNA Polymerase is based on the use of thermolabile blocking groups acting over the amino-acids residues involved in enzyme polymerization. Full enzyme activity is recovered after a short activation time during the initial denaturation step.
This formulation provides the following features to our Hot Start DNA Polymerase:
- Enhanced enzyme specificity and sensitivity.
- Increase the obtained yield.
- Reduce mispriming and primer-dimer formation.