TRUPCR® EBV Virus Detection Kit
The Epstein–Barr virus (EBV), also called human herpesvirus 4 (HHV-4), is one of eight known human herpes virus types in the herpes family, and is one of the most common viruses in humans. EBV is found all over the world. Most people get infected with EBV at some point in their lives. EBV spreads most commonly through bodily fluids, primarily saliva. EBV can cause infectious mononucleosis, also called mono, and other illnesses.
An illness associated with fever, sore throat, swollen lymph nodes in the neck, and some times an enlarged spleen. It is also known as human herpesvirus 4. Although EBV can cause mononucleosis, not everyone infected with the virus will get mononucleosis. White blood cells called B cells are the primary targets of EBV infection.
During TRUPCR® PCR, forward and reverse primers hybridize to a specific sequence product. A probe, which is contained in the same reaction mixture and which consists of an oligonucleotide labeled with a 5'-reporter dye and a downstream, 3'-quencher dye, hybridizes to a target sequence with in the PCR product. A Taq polymerase which possesses 5' - 3' exonuclease activity cleaves the probe. The reporter dye and quencher dye are separated upon cleavage, resulting in an increase in fluorescence for the reporter. Thus, the increase in fluorescence is directly proportional to the target amplification during PCR