TRUPCR® HPV High Risk Detection Kit
The TRUPCR® HPV High Risk Detection Kitis a Real-Time Amplification test for the qualitative detection of Human Papilloma Virus DNA in clinical samples. It is intended for the detection of 14 HPV high/intermediate-risktypes (16/18/31/33/35/39/45/51/52/56/58/59/66/68) in a real-time PCR based on the hydrolysis probe technology. HPV infections are among the most common sexually transmitted infections. More than one hundred different HPV genotypes have been identified, among which over forty infect mucosal and genital epithelia. Genital HPV genotypes are generally classified into high risk (HR) and low risk (LR) groups based on their carcinogenic potential. HR HPV genotypes are associated with invasive cervical cancer or its immediate precursor (high-grade squamous intraepithelial lesion, cervical intraepithelial neoplasia or carcinoma in situ). The TRUPCR® HPV High Risk Detection Kit is based on amplification of conserved region by primer and probes specific for HPV subtypes 16/18/31/33/35/39/45/51/52/56/58/59/66 and 68. In this kit there are six independent reactions running in parallel in 5 tubes: the first detects HPV 16 (FAM channel) and endogenous internal control DNA (IC) (HEX) which allows excluding unreliable results; second detects HPV18 (HEX channel); third tube detects HPV 31, 33, 35 and 39 (FAM); fourth tube detects HPV 45, 52, 56 and 66 (FAM); and fifth tube detects HPV 51, 58, 59 and 66 (FAM) high risk variants.