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3B DNA Polymerase

3B DNA Polymerase is a modified thermostable recombinant DNA polymerase from the thermophilic bacterium Thermus sp. expressed in E. coli. The general characteristics of 3B DNA Polymerase make the enzyme suitable for applications requiring a highly thermostable and processive enzyme capable of synthesizing DNA strands at elevated temperatures in amplification or similar reactions (e.g. primer extension), thus resolving the most complex secondary structures.
Due to its processivity and accuracy 3B DNA Polymerase allows the generation of long templates with a base misincorporation rate (1-10 x 10-6 bp) lower than most commercial Taq DNA polymerases.
The procedure employed for the purification of thermostable enzymes is proprietary of 3B BlackBio Biotech India Limited. It involves a simple and non chromatographic procedure which renders a top high yield and quality enzyme.
3B DNA Polymerase (1U/µl, 3U/µl and 5U/µl) comes with the choice of a Standard Reaction Buffer including 20 mM MgCl2 or a reaction buffer without MgCl2 (Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.

Specification:

Buffers Supplies with two different buffers combination and separate MgCl2 to allow PCR for wide range of magnesium concentration
Stability Stable at higher temperature
Application Standard PCR and Real Time PCR, Generates PCR products with 3'-dA overhangs
DNA Labelling Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Ordering Information:

3B DNA Polymerase (1U/µl)

CAT. NO. PRODUCT FORMAT
3B001 3B Recombinant DNA Polymerase (1U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 500U
3B002 3B Recombinant DNA Polymerase (1U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 1000U
3B005 3B Recombinant DNA Polymerase (1U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 500U
3B006 3B Recombinant DNA Polymerase (1U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 1000U

3B DNA Polymerase (3U/µl)

CAT. NO. PRODUCT FORMAT
3B021 3B DNA Polymerase (3U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 500U
3B022 3B DNA Polymerase (3U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 1000U
3B025 3B DNA Polymerase (3U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 500U
3B026 3B DNA Polymerase (3U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 1000U

3B DNA Polymerase (5U/µl)

CAT. NO. PRODUCT FORMAT
3B009 3B DNA Polymerase (5U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 500U
3B010 3B DNA Polymerase (5U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 1000U
3B014 3B DNA Polymerase (5U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 500U
3B015 3B DNA Polymerase (5U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 1000U

3B Hot Start DNA POLYMERASE

3B Hotstart DNA Polymerase has been designed and developed to overcome the problems due to non-specific priming, primer- dimer formation or other unwanted reactions occuring at low temperature during the PCR process.
The technology behind 3B Hot Start DNA Polymerase is based on the use of thermolabile blocking groups acting over the amino-acids residues involved in enzyme polymerization. Full enzyme activity is recovered after a short activation time during the initial denaturation step.

This formulation provides the following features to our Hot Start DNA Polymerase:

  • Enhanced enzyme specificity and sensitivity.
  • Increase the obtained yield.
  • Reduce mispriming and primer-dimer formation.

Ordering Information:

3B Hot Start DNA Polymerase (1U/µl)

CAT. NO. PRODUCT FORMAT
3B035 3B Hotstart DNA Polymerase (1U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 250U
3B036 3B Hotstart DNA Polymerase (1U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 500U
3B037 3B Hotstart DNA Polymerase (1U/µl) – Standard 10X Reaction Buffer with 20mM MgCl2 1000U
3B041 3B Hotstart DNA Polymerase (1U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 250U
3B042 3B Hotstart DNA Polymerase (1U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 500U
3B043 3B Hotstart DNA Polymerase (1U/µl) – 10X Reaction Buffer MgCl2 Free + 50mM MgCl2 1000U
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