TRUPCR® UTI AST Panel Kit is a multiplexed Real Time PCR kit which is intended for detection of antimicrobial sensitivity of uropathogen(s). It includes five tubes targeting fourteen antimicrobial resistance genes representing different antibiotic classes for antimicrobial susceptibility testing to accurately diagnose the resistance profile and suggest a definitive treatment recommendation for each UTI patient. Endogenous internal control is included in each tube. This assay is based on TaqMan hydrolysis probe which allows higher specificity and sensitivity. The targets are detected with the help of four different dyes (FAM/Green, HEX/Yellow, Texas Red/Orange and Cy5/Red).
Key Features:
-
- Fastest Assay (Run time: Less than 1 hour)
- AST profiling targeting 14 genes with 5 different classes of antibiotics
Technical Specification:
- Sample Type – Urine Specimen /Cultured isolates
- UNG is included in the master mix to avoid carry over contamination
- Reaction Volume – 20 µl in each tube
- Room temperature reaction setup
- LoD: 1 x 10³ Copies/Reaction
- Clinical Validation – Extensively validated on over 500 samples
- Compatible Instruments – CFX96, QuantStudio® 5, ABI 7500, Rotor-Gene Q
Target Antibiotic Resistance Gene:
| Tube* | Antibiotic | Target Gene | Dye |
|---|---|---|---|
| 1 | Sul2 | FAM | |
| SXT | dfrA1 | Texas Red | |
| dfrA5 | Cy5 | ||
| 2 | blaCTX-M | FAM | |
| ESBLs | blaTEM | Texas Red | |
| blaSHV | Cy5 | ||
| 3 | blaKPC | FAM | |
| Carbapenems set 1 | blaNDM | Texas Red | |
| blaVIM | Cy5 | ||
| 4 | blaOXA-48 | FAM | |
| Carbapenems set 2 | blaIMP | Texas Red | |
| 5 | Methicillin | mecA | FAM |
| Vancomycin | vanA | Texas Red | |
| vanB | Cy5 |
*Endogenous internal control (labeled with HEX) is included in each tube to avoid false negative results
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B343 | TRUPCR® UTI AST Panel Kit | 48 Reactions |
| 3B344 | TRUPCR® UTI AST Panel Kit | 96 Reactions |