The TRUPCR® ROS1 Gene Fusions Detection Kit is intended for the qualitative detection of 14 ROS1 gene fusions in human NSCLC formalin-fixed paraffin- embedded (FFPE) tissue samples using real time PCR system. The human ABL1 gene serves as an internal control for human nucleic acid, also included in this kit.
Principle and Procedure:
It has two-step protocol in which total RNA is reverse- transcribed, and the generated cDNA is amplified by PCR using a pair of specific primers and a specific internal double-dye probe of ROS1 and endogenous control gene ABL1 to monitor the quality and quantity of extracted RNA.
Key Features:
- Detection of 14 ROS1 fusion gene transcripts in single test.
- One step kit for fast (~ 80 min) screening of ROS1 status of patient.
- All inclusive kit: The Assay includes cDNA preparation components and all the PCR components
- including PCR Pre-mix / mastermix for optimized results.
| Tube.No. | ROS1 Spliced Exon | Spliced Gene & Exon | Remarks |
|---|---|---|---|
| 1 | ROS1 exon32 |
SLC34A2 exon4 & & SLC34A2 exon14del CD74 exon6 SDC4 exon2 SDC4 exon4 |
It detects 5 fusions but does not distinguish between them. |
| 2 | ROS1 exon34 |
SLC34A2 exon4 & & SLC34A2 exon14del CD74 exon6 SDC4 exon4 EZR exon10 |
It detects 5 fusions but does not distinguish between them. |
| 3 | ROS1 exon35 |
TPM3 exon8 LRIG3 exon16 GOPC exon8 |
It detects 3 fusions but does not distinguish between them. |
| 4 | ROS1 exon36 | GOPC exon4 | It detects 1 fusion. |
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B1349 | TRUPCR® ROS1 Gene Fusions Detection Kit | 24 Reactions |
| 3B1350 | TRUPCR® ROS1 Gene Fusions Detection Kit | 48 Reactions |