TRUPCR® NPM1 Mutation Quantitative Kit is an in-vitro nucleic acid amplification assay for the qualitative detection and quantification of NPM1 mutant allelic burden in clinical samples.
In this assay, extracted RNA is subjected to a separate real-time reverse transcription-polymerase chain reaction (RT-PCR) procedures to detect and quantitate NPM1 mutations (mutA, mutB and mutD) in exon 12 by real- time PCR. An additional amplification for the ABL1 gene is performed as a control for sample RNA quality and as a reference for relative quantification.
Three types of NPM1 mutation variants quantified by this kit are given below:
- NPM1 mutation A (c.860_863dupTCTG),
- NPM1 mutation B (c.863_864insCATG),
- NPM1 mutation D (c.863_864insCCTG
Key Features:
- Reliable and sensitive detection and differentiation of NPM1 A, B and D mutations.
- Reliable and specific quantification of NPM1 A, B, and D mutations
- The kit offers sensitivity to detect up to 50 copies of mutant transcripts.
- All the reagents for cDNA chemistry and Real-time PCR are included in the kit
- It is compatible with various Real Time PCR instruments.
- Easy-to-use, rapid, reliable, comprehensive and cost-effective tests.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B325 | TRUPCR® NPM1 Mutation Quantitative Kit | 24 Reactions |
| 3B326 | TRUPCR® NPM1 Mutation Quantitative Kit | 48 Reactions |