MYD88 Mutations Detection

Test Overview

The TRUPCR® MYD88 Mutation Detection Kit is an in vitro diagnostic test intended for the qualitative detection of L265P mutation in c.794T>C in myeloid differentiation factor 88 (MYD88) gene from tumor tissue DNA (fresh, frozen or formalin fixed paraffin-embedded tissue)/bone marrow or peripheral blood. Results are intended to aid the clinician in classifying patients among Lymphoplasmacyticlymphoma/Waldenstrom Macroglobulinemia (LPL/WM) and nonHodgkin lymphoma and carry out treatment planning.

Principle and Procedure:

The TRUPCR®MYD88 Mutation Detection Kit is based on allele specific amplification and is achieved by ARMS PCR. The kit is designed to selectively amplify mutant specific sequences in samples that contain a mixture of wild-type and mutated DNA. The TRUPCR®MYD88 Mutation Detection Kit is a single tube kit where the detection is achieved in multiplex reaction using fluorescent probes labeled with FAM (L265P mutation in c.794T>C) and VIC/HEX (reference: a region of MyD88 gene without any known Mpolymorphism/mutation).

Key Features:

• The kit allows highly sensitive and specific diagnosis of MyD88  Mutation in single run.
• The kit offers sensitivity to detect as low as 0.5% allele of MyD88 L265P.
• All inclusive kit: The Assay includes all the PCR components including PCR Pre-mix / mastermix for optimized results.
• Positive control is included in the kit for reliable results