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LADDERS

We offer a broad range of ladders with fragment sizes ranging from 50bp to 10 kb, for accurate analysis of linear double-stranded DNA in agarose or polyacrylamide gels. DNA ladders are made of unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield different fragments,suitable for use as molecular weight standards for gel electrophoresisDNA ladders are stable during prolonged incubation at room temperature and multiple freeze-thaw cycles.

1 kb DNA Ladder

The 1 kb DNA Ladder is prepared from vector DNA digested to completion with appropriate restriction enzymes to yield bands ranging from 250 bp to 10 kb, suitable for use as molecular weight standards for agarose gel electrophoresis. The ladder is composed to 14 chromatography-purified individual DNA fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500 and 250. The 1000, 3000 and 6000 bp fragments have increased intensity to serve as reference bands.


100bp DNA Ladder

The 100 bp DNA Ladder is prepared from plasmid DNA digested to completion with appropriate restriction enzymes to yield bands ranging from 100 bp to 1500 bp, suitable for use as molecular weight standards for agarose and polyacrylamide gels. The ladder is composed to 11 chromatography purified individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. The 500bp and 1500bp fragment has increased intensity to serve as a reference band.


50bp DNA Ladder

3B 50 bp DNA Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. It is a unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes. The ladder is composed of seventeen fragments, suitable for use as molecular weight standards for gel electrophoresis. The DNA includes fragments ranging from 50-1500 base pairs. It contains two reference bands (500 and 250bp ) for easy orientation. The ladder is dissolved in TE buffer.

Ordering Information:

LADDERS

CAT. NO. PRODUCT FORMAT
3B194 3B 100bp DNA Ladder 50 µg
3B195 3B 1Kb DNA Ladder 50 µg
3B196 3B 50bp DNA Ladder 50 µg
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