TRUPCR® SARS-CoV-2 RT qPCR Kit (V-3.2) is an in vitro nucleic acid amplification assay for the qualitative detection of SARS-CoV-2 RNA from a suspected infection patient’s sample through Real-Time Polymerase chain reaction (PCR).
The TRUPCR® SARS-CoV-2 RT qPCR kit (V-3.2) composed of single tube assays which include primers and probes specific to: 1) E gene – for the detection of the Sarbecovirus (of Genus B-betacoronavirus (B-βCoV) on Texas Red/ROX/Orange channel 2) RdRp gene & N gene – dual targets for the detection of SARS-CoV-2, on FAM/Green channel and 3) RNaseP gene, endogenous internal control on HEX/ROX/Yellow channel.
Key Features:
- Recommended by ICMR, approved by NIV and licenced by CDSCO
- Single tube assay
- Assay design based on WHO recommended primers and probes
- 3 gene targets, E gene for screening along with RdRp & N gene for confirmation of SARS-CoV-2 RNA
detection - RNaseP gene as endogenous internal control is included in the kit to avoid false negativity
- Takes advantage of the “hot start” technology to minimizing non-specific reactions and assuring
maximum sensitivity & specificity. - Detection limit of this assay is 6 copies/μl
- Calibrated with reference material, AcroMetrix Coronavirus 2019 (COVID-19) RNA Control (Thermo
Fisher Scientific, USA, Ref: 954519) - Single step detection from RNA sample
- Easy work flow & compatible with various real time PCR instruments.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B306 | TRUPCR® SARS-CoV-2 RT qPCR Kit | 100 Reactions |
| 3B307 | TRUPCR® SARS-CoV-2 RT qPCR Kit | 200 Reactions |
Publications :
- Ahmed, Faiq, et al. "Retrospective Study of B Lymphoblastic Leukemia to Assess the Prevalence of TEL/AML1 in South India: A Study of 214 Cases and Review of Literature." Indian Journal of Medical and Paediatric Oncology (2022).
- Mehta, Priyanka, et al. "Respiratory co-infections: modulators of SARS-CoV-2 patients’ clinical sub-phenotype." Frontiers in Microbiology (2021): 1268.
- Iliescu, Florina Silvia, et al. "Point-of-Care Testing—The Key in the Battle against SARS-CoV-2 Pandemic." Micromachines 12.12 (2021): 1464.
- Gupta, Abhishek, et al. "Oral dysbiosis and its linkage with SARS-CoV-2 infection." Microbiological research 261 (2022): 127055.
- Ghosh, Susmita, et al. "Semen proteomics of COVID-19 convalescent men reveals disruption of key biological pathways relevant to male reproductive function." ACS omega 7.10 (2022): 8601-8612.
- Das, Parijat, et al. "COVID diagnostics by molecular methods: A systematic review of nucleic acid based testing systems." Indian Journal of Medical Microbiology 39.3 (2021): 271-278.
- Sharma, Ravi Prakash, et al. "Clinico epidemiological profile of Omicron variant of SARS CoV2 in Rajasthan." medRxiv (2022): 2022-02.
- Devi, Priti, et al. "Increased abundance of Achromobacter xylosoxidans and Bacillus cereus in upper airway transcriptionally active microbiome of COVID-19 mortality patients indicates role of co-infections in disease severity and outcome." Microbiology Spectrum 10.3 (2022): e02311-21.
- Sharma, Ravi P., et al. "Genomic profile of SARS-CoV-2 Omicron variant and its correlation with disease severity in Rajasthan." Frontiers in Medicine 9 (2022): 888408.
- Pandit, Priyanka, et al. "A case of breakthrough infection with SARS-CoV-2 Delta derivative and reinfection with Omicron variant in a fully vaccinated health care professional." Journal of Infection 85.1 (2022): e15-e17.
- Ayaz, Akif, et al. "A pooled RT-PCR testing strategy for more efficient COVID-19 pandemic management." International Journal of Infectious Diseases 116 (2022): 1-6.
- Devi, Priti, et al. "Transcriptionally active nasopharyngeal commensals and opportunistic microbial dynamics define mild symptoms in the COVID 19 vaccination breakthroughs." PLoS Pathogens 19.2 (2023): e1011160.
- Sigamani, Alben, et al. "An Oral Galectin Inhibitor in COVID-19—A Phase II Randomized Controlled Trial." Vaccines 11.4 (2023): 731.
- Wani, Hima, et al. "Wastewater-Based Epidemiology of SARS-CoV-2: Assessing Prevalence and Correlation with Clinical Cases." Food and Environmental Virology (2023): 1-13.