TRUPCR® MRSA Real-Time PCR Detection kit is an in vitro nucleic acid amplification test for the qualitative detection of Staphylococcus aureus and its Methicillin-Resistant status from various sources of clinical samples using Real time PCR system. The MRSA detection consists in amplification of two specific loci (the nuc gene which is specific for Staphylococcus aureus , and the resistance gene mecA) and in measurement of fluorescence increase. In this kit there are three independent reactions running in parallel in each tube: the first detects Staphylococcus aureus (FAM channel), the second detects Methicillin-Resistant Staphylococcus aureus (HEX channel), and the third detects internal control (IC) DNA (Tex Red channel) which allows excluding unreliable results.
Key Features:
- The test allows highly sensitive qualitative detection of Staphylococcus aureus and its Methicillin-Resistant status
- PCR kit uses “hot-start” Taq-polymerase enzyme which greatly reduces the frequency of nonspecifically primed reactions.
- This test is validated on different real time machines (Applied Biosystems 7500/7500 Fast DX/StepOne/StepOne Plus Real-Time PCR System, Biorad CFX96™, LineGene 9600 / 9600 Plus, QuantStudio™ 3/5 Real-Time PCR System, Rotor-Gene 3000/Q).
- Easy workflow with ready-to-use reagents.
- Simple reaction setup and data interpretation, no special personnel training required.
- This assay can be done with routine molecular lab set-up and most of the existing real-time PCR instruments.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B1283 | TRUPCR® MRSA Real-Time PCR Detection kit | 48 Reactions |
| 3B1284 | TRUPCR® MRSA Real-Time PCR Detection kit | 96 Reactions |
