NAAT

The TRUPCR® NAAT Kit (RUO*) is Nucleic Acid Amplification Test for the qualitative detection of Hepatitis B viral DNA, Hepatitis C viral RNA and Human Immunodeficiency type-1/type-2 viral RNA in human serum or plasma (EDTA) using Real Time PCR technique. TRUPCR® NAAT Kit (RUO) is an one step real time reverse transcription PCR assay in which RNA templates are first reverse transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification.

In this kit there are five independent reactions are running in parallel in each tube: the first detects HIV type1/type-2 viral targets in FAM/Green channel, second detects HCV viral targets in HEX/Yellow channel, third detects HBV viral targets in Orange/Tex Red/ROX channel and forth detects RnaseP gene as endogenous internal control in Red/Cye5 channel which allows excluding unreliable results. The analytical sensitivity** of the TRUPCR® NAAT Kit is confirmed by WHO International reference material. The TRUPCR® NAAT Kit demonstrated the ability to reproducibly detect the presence of HBV at the level of 2.5 IU/ml, HCV at the level of 25 IU/ml and HIV type-1 & type-2 at the level of 100 IU/ml (*RUO = Research Use Only, **when 0.5 ml volume of individual patient sample is used for extraction).