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The TRUPCR® NAAT Kit (RUO*) is Nucleic Acid Amplification Test for the qualitative detection of Hepatitis B viral DNA, Hepatitis C viral RNA & Human Immunodeficiency type-1 viral RNA in human serum or plasma (EDTA) using Real Time PCR technique.

TRUPCR® NAAT Kit (RUO) is a two step real time reverse transcription PCR assay in which samples are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification. In this kit there are two independent reactions running in parallel in each tube: the first detects viral targets (FAM/Green channel) and second detects endogenous internal control (IC) DNA (HEX/Yellow channel) which allows excluding unreliable results.

The analytical sensitivity** of the TRUPCR® NAAT Kit is confirmed by WHO International Standard. The TRUPCR® NAAT Kit demonstrated the ability to reproducibly detect the presence of HBV at the level of = 2.5 IU/ml, HCV at the level of = 25 IU/ml and HIV-1 at the level of = 100 IU/ml (*RUO = Research Use Only, **when 0.5 ml volume of individual patient sample is used for extraction).

Key Features:

  • Rapid and accurate detection of HBV, HCV & HIV-1 infection.
  • Takes advantage of the "hot start" technology to minimizing non-specific reactions and assuring maximum sensitivity & specificity.
  • Takes advantage of the uracil DNA glycosylase (UDG) in MasterMix to eliminating possible contamination of the PCR with amplification products.
  • Analytical sensitivity is determined by WHO standards.
  • Compatible with various real time PCR instruments.
  • Endogenous Internal Control included in the kit in each tube to avoid any false negative result.
  • Available in different pack sizes and extraction formats

Ordering Information:

3B251 TRUPCR®NAAT Kit 48 Rxn
3B252 TRUPCR®NAAT Kit 96 Rxn
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