TRUPCR® MTB DNA Extraction Kit is intended for the extraction and purification of DNA from M.tuberculosis spp. in human samples (sputum, BAL, PUS, tissues and other body fluids).TRUPCR® MTB DNA Extraction Kit is based on the advanced silica binding technology.
The DNA extraction procedure includes 4 steps:
Heat Lysis- Lysis of the bacterial cell wall at high temperature and release of DNA.Binding- Adsorption of the DNA on to the membrane.
Washing- Removal of residual contaminants.
Elution- Elution of pure genomic DNA
Key Features:
- Based on Silica column technology, reduces the hands on procedure time.
- TRUPCR® MTB DNA Extraction kit is validated on all clinical specimens including sputum, PUS, BAL, tissue, CSF and other body fluids.
- Phenol chloroform free method.
- High binding capacity of the column provides high yield of purified DNA.
Specification:
| Technology | Silica Column |
| Sample Type | Sputum, Pus, BAL, Tissue, Urine, Body fluids and tissue |
| Application | PCR and Real Time PCR |
| Elution Volume | 40-50 µl |
| Compatible with | TRUPCR® HPV 16/18 Kit and TRUPCR® Leptospira Detection Kit, |
| Processing | Manual (Centrifugation) |
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B268E | TRUPCR MTB DNA Extraction Kit | 50 Reactions |
| 3B269E | TRUPCR MTB DNA Extraction Kit | 100 Reactions |