AST Panel Kit

NEED

The discovery and use of antibiotics are two of the mostsignificant breakthroughs in twentieth-centurymedicine—leading to dramatic reductions in humanmorbidity and mortality. One emerging problem withantibiotics is the development of antibiotic-resistant bacteria.Antimicrobial resistance (AMR) occurs when microbes evolvemechanisms that protect them from the effects ofantimicrobials. Infections due to AMR cause millions ofdeaths each year. So, antibiotic resistance is one of the mosturgent threats to public health. Effective treatment ofinfection depends on the accurate identification of thepathogen(s) and the correct choice of antibiotic(s). Henceaccurate antimicrobial susceptibility (AST) profiling of a pathogen is very important.

Test Overview

TRUPCR® AST Panel Kit is an in vitro nucleic acid amplification assay for the qualitative detection & differentiation of antimicrobial resistance genes encoding resistance to antibiotics - Trimethoprim/Sulfamethoxazole (SXT), Extended-spectrum beta-lactams (ESBLs), Carbapenems, Vancomycin and Methicillin using Real Time PCR System. It includes five tubes targeting fourteen antibiotic resistant genes. This assay is based on TaqMan hydrolysis probe which allows higher specificity and sensitivity. 

The targets are detected with the help of four different dyes (FAM/Green, HEX/Yellow, Texas Red/Orange and Cy5/Red). This kit can be used with the extracted genomic DNA from clinical samples or cultured isolates.