Test Overview
TRUPCR® Carbapenem Resistance Detection Kit is an in vitro nucleic acid amplification assay for the qualitative detection and differentiation of the blaKPC (KPC-Klebsiella pneumoniae carbapenemase), blaNDM (NDM-New Delhi Metallo-beta-lactamase), blaVIM (VIM-Verona integron-mediated metallo-beta-lactamase), blaOXA-48-like (OXA-48-Oxacillinase-48-like), and blaIMP (IMP-Imipenemase metallo-beta-lactamase) gene sequences associated with carbapenem-non-susceptibility on Real-Time PCR. The TRUPCR® Carbapenem Resistance Detection Kit is not intended to guide or monitor treatment for carbapenem-non-susceptible bacterial infections. A negative result from TRUPCR® Carbapenem Resistance Detection Kit does not prevent the presence of other resistance mechanisms. The results from the TRUPCR® Carbapenem Resistance Detection Kit must be interpreted within the context of all relevant clinical and laboratory findings.
An Internal Control has been integrated into the kit in order to check PCR inhibition.
TECHNICAL SPECIFICATIONS
- The test allows highly sensitive qualitative detection and differentiation of the blaKPC (KPC-Klebsiella pneumoniae carbapenemase), blaNDM (NDM-New Delhi Metallo-beta-lactamase), blaVIM (VIM-Verona integron-mediated metallo-beta-lactamase), blaOXA-48-like (OXA-48-Oxacillinase-48-like), and blaIMP (IMP-Imipenemase metallo-beta-lactamase) gene sequences associated with carbapenem-non-susceptibility on Real-Time PCR.
- PCR kit uses “hot-start” Taq-polymerase enzyme which greatly reduces the frequency of nonspecifically primed reactions.
- Sample Type – Respiratory specimens (BAL/Tracheal aspirate/Sputum/Nasopharyngeal aspirate), Rectal swabs and bacterial culture use as a sample for this test.
- This test is validated on different real time machines (Applied Biosystems™ 7500/7500 Fast Dx, Applied Biosystems™ QuantStudio® 3 and 5, Rotor-Gene Q, Bio-Rad CFX96)
- Easy workflow with ready-to-use reagents.
- Simple reaction setup and data interpretation, no special personnel training required.
- This assay can be done with routine molecular lab set-up and most of the existing real-time PCR instruments.
- Internal Control integrated into the kit in order to check PCR inhibition
About Carbapenem Resistance:
Carbapenem resistance is a major and an on-going public health problem globally. It occurs mainly among Gram-negative bacteria. Infections by such pathogens pose a serious threat to hospitalized patients due to resistance of all beta-lactam antibiotics as well as co- resistance to most other antibiotics, leaving very few treatment options which are associated with clinical and economic consequences. Culture is the reference method for detection, requires at least a turn-around time of between 2-3 days which will be critical in emergency and ICU patients when the time available for intervention is limited. New CLSI recommendations aim to speed up detection and improve treatment outcomes for Carbapenem resistance through use of a molecular diagnostic test.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B289 | TRUPCR® Carbapenem Resistance Detection Kit | 48 Reactions |
| 3B290 | TRUPCR® Carbapenem Resistance Detection Kit | 96 Reactions |
Publications:
- Partha Guchhait, Nairita Choudhuri, et al. “Improved diagnostic stewardship in carbapenem-resistant Enterobacterales gene detection helps in early initiation of targeted therapy”
- Gaurav Verma, Subham Ravi Nayak, et al. “Detection of Carbapenem-Resistant Genes Among Clinical Isolates of Acinetobacter baumannii in a Tertiary Care Hospital”
