Meningitis Panel

Test Overview

TRUPCR® Meningitis Panel Kit is an in vitro nucleic acid amplification assay for the qualitative detection and differentiation of 12 viruses and 9 bacterial nucleic acid on Real-Time PCR. The kit targets Human adenovirus, Enterovirus Human Parechovirus, Herpes simplex virus 1, Herpes simplex virus 2, Mumps Virus, Human Parvovirus B19, Epstein-Barr Virus, Varicella Zoster virus, Human Cytomegalovirus, Human Herpesvirus 6, Human Herpesvirus 7, Haemophilus influenza, Neisseria meningitides, Escherichia coli, Streptococcus agalactiae, Streptococcus pneumonia, Listeria monocytogenes, Non-tuberculosis Mycobacterium, Mycobacterium tuberculosis complex, and Cryptococcus neoformans/gattii.

Endogenous internal control is incorporated into all the tubes in kit to verify the quality of samples, quality of extracted nucleic acid, amplification procedure and possible presence of inhibitors, which may cause false negative results. This design of the kit makes it highly reliable. This assay is based on oligonucleotide hydrolysis principle which allows higher specificity and sensitivity. This is a single step detection assay where different targets are detected with the help of 4 different dyes (FAM/Green, HEX/VIC/Yellow, Texas Red/ROX/Orange & Cy5/Red).

Advantages of TRUPCR® Meningitis Panel Kit:

• Multiplex assay for detection & differentiation of 12 viruses and 9 bacterial nucleic acid
• Higher sensitivity and specificity
• Provided with endogenous internal control to exclude unreliable results
• Easy workflow & compatible with most of the real-time PCR equipment.
• All the reagents required for the test included in the kit.

TECHNICAL SPECIFICATIONS

• Sample Type – Extracted total nucleic acid from cerebrospinal fluid (CSF) samples of human origin
• Clinical Validation – Validated on more than 500 clinical samples
• Target Regions – Conserved regions of the genome of each pathogen
• Reaction Volume – 25 µl in each tube
• LOD Data: Viral pathogen - 700 IU/ml, Bacterial Pathogens – 500 CFU/ml
• Compatible Instruments – Applied Biosystems™ 7500, Applied Biosystems™ QuantStudio® 5, Rotor-Gene Q, Bio-Rad CFX96

About Meningitis:

Meningitis is a life-threatening condition caused by inflammation of the protective membranes surrounding the brain and spinal cord, most often due to bacterial and/or viral infections. Acute bacterial meningitis—primarily caused by Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae, and Streptococcus agalactiae—is among the most severe forms, capable of triggering sudden epidemics, leading to death within 24 hours, and leaving one in five survivors with lifelong disabilities. In 2019 alone, meningitis was responsible for approximately 250,000 deaths worldwide. While bacterial meningitis is widely recognized for its severity, viral pathogens are equally important contributors to neuroinfectious diseases and warrant equal clinical attention. Enteroviruses, human herpesviruses (HHV), HSV-1, HSV-2, Epstein-Barr virus, varicella-zoster virus, cytomegalovirus, HHV-6, and HHV-7—can contribute to meningitis or encephalitis, particularly in pediatric and immunocompromised patients. Given the rapid progression and severe outcomes of both bacterial and viral neuroinfections, early and accurate detection of causative pathogens is vital. Timely diagnosis through Real-Time PCR (considered as the gold standard), not only improves patient survival and recovery but also enables targeted antiviral or antibiotic therapy, reducing long-term complications and preventing outbreaks.