truNGS® Pan-Myeloid Panel Kit

The TruNGS® Pan-Myeloid Panel kit has been developed to identify SNVs, Indels, and RNA fusions across 95 clinically significant genes, offering comprehensive exon coverage for 20 genes, hotspot analysis for 34 key marker genes, and fusion detection in 35 genes linked to major myeloid disorders such as AML, MPN, MDS, CML, CMML, JMML, and others.

The assay also detects hotspot mutations in 6 PGx genes linked to chemotherapy response, offering guidance for personalized dose adjustments.

The protocol involves two major steps:

(A) Library preparation (input DNA fragmentation, adapters ligation, PCR amplification of individual libraries); and

(B) Hybrid Capture and target enrichment (library pooling, probes hybridization, capture, and post- capture PCR amplification)

For use on Illumina and MGI platforms only