TRUPCR® HSV1/2 Real-Time PCR Kit
TRUPCR® HSV 1/2 Real-Time PCR Detection Kit is in vitro nucleic acid amplification assay for the qualitative single tube detection of Herpes Simplex Virus-1 (HSV-1) and Herpes Simplex Virus-2 (HSV-2) from clinical samples. Herpes Simplex Virus 1 and 2 (HSV-1&2) are both members of the herpes virus family, Herpesviridae. HSV-1 and HSV-2 have relatively large double-stranded DNA genomes. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterized by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis; primary HSV-2 infection occurs as, among others, vulvovaginitis, meningitis and generalized herpes in newborns. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. Even more severe are the recurrent forms of keratoconjunctivitis and meningitis. The infection is contagious, mainly during an epidemic.
TRUPCR® HSV 1/2 Real-Time PCR Detection Kit is based on amplification of conserved region of the viral genome. In this kit there is a multiplex reactions are running in parallel in single tube: the first detects HSV-1 in FAM channel, second detects HSV-2 in HEX channel and third detects internal control (IC) DNA in TexRed channel which allows excluding unreliable results.