Test Overview
Chronic myeloid leukemia (CML) is characterized by the consistent involvement of the Philadelphia chromosome (Ph) and the BCR-ABL fusion gene, which derives from the reciprocal translocation t(9;22)(q34;q11) between chromosome 9 and 22. In almost all CML patients, the breakpoint in the BCR gene involves the Major breakpoint cluster region (M-bcr). The position of the breakpoint within the M-bcr, after exon b2 (e13) or exon b3 (e14) determines two main types of the fused BCR-ABL mRNA defined as b2a2 and b3a2 transcripts differing by 75 nucleotides. These transcripts encode two 210-kDa tyrosine kinase proteins (p210 BCR-ABL), which differ by 25 amino acids respectively. The impact of M-bcr breakpoint position on disease phenotype and its prognosis has been a subject of controversies for a long time. Several reports have suggested that the type of the chimeric mRNA (b2a2 or b3a2) is associated with differences in the clinical and hematological characteristics of CML patients and prognosis1.
The TRUPCR BCR-ABL1 b2a2/b3a2 kit is a real time PCR assay which detects BCR-ABL1 fusion gene transcripts P210 (e13a2, e14a2), P190 (e1a2), P230 (e19a2) and also differentiate between e13a2 (b2a2) and e14a2 (b3a2) transcript in bone marrow or peripheral blood samples of chronic myeloid leukemia (CML) or acute lymphoblastic leukemia (ALL) or acute myelogenous leukemia (AML).
Key Features:
- Simultaneous detection of Major, Minor & Micro BCR ABL1 fusion transcript.
- Differentiation of b2a2/b3a2 transcript of Major BCR ABL1 fusion on real time PCR.
- All the reagents required for the test included in the kit.
- Compatible with various real time PCR instruments.
- Higher sensitivity and specificity with easy workflow and quick analysis.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B1250 | TRUPCR® BCR-ABL1 b2a2/b3a2 Diffrentiation Kit | 48 Reactions |
Publications :
- Singh, Neetu, et al. "Differential genomics and transcriptomics between tyrosine kinase inhibitor-sensitive and-resistant BCR-ABL-dependent chronic myeloid leukemia." Oncotarget 9.54 (2018): 30385.
- Chadha, Ritu, et al. "Cytogenetic Risk Stratification of B-Acute Lymphoblastic Leukemia and Its Correlation with Other Prognostic Factors." Indian Journal of Hematology and Blood Transfusion 39.1 (2023): 141-145.
- Dey, Samya, et al. "Deep sequencing reveals the spectrum of BCR-ABL1 mutations upon front-line therapy resistance in chronic myeloid leukemia: An Eastern-Indian cohort study." Cancer Treatment and Research Communications 33 (2022): 100635.