VRE Detection

Disease Overview

Enterococcus spps. are normally present in the human intestines, but recently they are recognized as major pathogens causing urinary tract infections and sepsis by nosocomial infection. Entensive antibiotic treatments has caused Enterococcal strains such as E. faecalis or E. faecium to become resistant to vancomycin, which is commonly used in the treatment of Enterococci infection. Acquired resistance to vancomycin Resistant Entercocci (VRE) poses a major public health problem since it was first reported. VRE are among the most common resistant pathogens frequently causing healthcare associated infections and a growing concern for health care professionals. A fast and effective identification of VRE is essential to prevent its nosocomial transmission.

Test Overview

TRUPCR® VRE Detection Kit is a multiplexed real time PCR kit which is intended for sensitive and specific detection of Enterococcus spp. (E. faecalis or E. faecium), vanA and vanB genes in clinical samples / cultured isolates. This is a single tube reaction targeting Enterococcus spp. and vancomycin resistance genes vanA and vanB along with an endogenous internal control. This assay is based on TaqMan hydrolysis probe which allows higher specificity and sensitivity.

Key Features:

• • Simultaneous identification of Enterococcus spp. and vancomycin resistance genes in one single tube
  • Sample type – Rectal Swab / Urine / cultured isolates
  • Run time less than 1 hour
  • Sensitive to detect upto 10 copies/reaction
  • UNG is included in the mastermix to avoid carry over contamination
  • Compatible with Applied BiosystemsTM 7500 series, Quantstudio® 5, Qiagen Rotor-Gene Q, Bio-Rad CFX96 and MIC PCR